Products | SPR Sensorchips

SPR Sensor chips

With SPR (Surface Plasmon Resonance) and diverse surface chemistries, scientists have discovered an invaluable tool with which to investigate molecular and cellular reactions in fields including immunology, molecular biology, cell biology and biochemistry, as well as many others.

The quality of the data obtained with this technology depends mainly on the sensor chip surface, as it is here that the biomolecular interaction takes place and is transduced into an optoelectronic signal.

XanTec bioanalytics provides laboratories worldwide with the ultimate in SPR biosensor chips via superior coating technologies. Our proprietary technology, developed over more than a decade ago, solves a number of critical issues usually associated with state of the art Self Assembled Monolayer (SAM) surface chemistry. The coatings are robust and prevent exposure of hydrophobic nanodomains or pinhole defects which can cause non-specific interactions. As the chemistry is extremely versatile, a large choice of topcoats is available to specifically address different experimental needs.

Not only do we feature the largest portfolio of sensorchip coatings and chemistries in the world, we offer the versatility to work on a number of different instruments and in varying formats. The chips fit into instruments of all major manufacturers. In addition, we provide customized sizes or can coat alternative substrate materials.

Compatible sensor chips and prisms
Biacore™
BioNavis™
Bruker™ / Sierra Sensors™
Sartorius™ / ForteBio™ / SensiQ™
Reichert SPR
BioRad™ ProteOn®
IBIS MX96® prisms
IBIS/Kinetic Evaluation Instruments™
Horiba™
Instrument compatibility of XanTec's SPR sensorchips/prisms. Other manufacturers are covered by OEM contracts.

Short brochure SPR chips

Chip selection guide

To ensure optimal results, XanTec offers an extensive range of different sensor chips spanning a variety of polymers, densities, thicknesses and capture molecules. The following charts may serve as orientation to find the optimal chip surface dependent on assay format, type of interaction and interacting (bio-) molecules.

Suggested hydrogel thickness by molecular weight of analyte
Molecular weight of Analyte (Da) <100 100 – 1000 1k-10k 10k-150k >150k
Recommended hydrogel thickness (nm) ≥500 500 - 200 200 - 50 50 - planar
Suggested hydrogel density by molecular weight of analyte
Molecular weight of Analyte (Da) <100 100 – 1000 1k-10k 10k-150k >150k
Recommended hydrogel density dense dense-medium medium medium-low low
Polymer 01a
Application (ligand – analyte)Suggested structure / polymer typeSuitable sensor chip(s)
Protein – protein, assayHydrogel, 100 – 500 nm, low - medium density. Linear polycarboxylate or carboxymethyldextran.CMD200M, CMD500L, HC200M, (HC1000M)
Protein – protein, kinetics2D surface or thin hydrogel with low density.CMDP, CMD50L, CMD50M, CMD200L, HC30M
Protein – DNA or polysaccharideHydrogel, 30 – 1000 nm, medium density. Linear polycarboxylate.HC30M, HC200M, HC1000M
Protein – peptide/small moleculeHydrogel, >500 nm, high density. Linear polycarboxylate or carboxylated polysaccharideCMD500M
HC1000M, HC1500M
Protein – virus, particle or bacteria (cells)Thin (50 nm) hydrogel, 2D surface, medium-high density.  Linear polycarboxylate or carboxymethyldextran.CMDP, CMD50M
HC30M
DNA – DNA, small molecules, peptidesHydrogel, > 200 nm, medium - high density. Streptavidin modified linear polycarboxylate or carboxymethyldextran.SAD500M, SAHC1000M
DNA – proteinHydrogel, 100 – 500 nm, low - medium density. Streptavidin modified carboxymethyldextran or linear polycarboxylate.SAD50M, SAD200L, SAD200M, SAD500L
SAHC200M
DNA – proteinHydrogel, 30 – 500 nm, low - medium density. Neutravidin derivatized carboxymethyldextran or linear polycarboxylate. May reduced non-specific binding for positively charged analytes.NAD50M, NAD200M, NAD500L
NAHC30M, NAHC200M
Click coupling
Especially Sensitive Ligands (e.g. Proteins with restrictions in buffer composition) and all other proteins
Azide modified linear polycarboxylate and carboxymethylated dextran with DBCO modified ligand – DBCO Labeling Kit
Preferable alternative to biotin/streptavidin
AZD50L, AZD200M
AZHC30M, AZHC200M, AZHC1500M
Cell, virus, particle, lipid bilayers – any species2D surface or thin (50 nm) hydrogel, medium-high density.  Linear polycarboxylate or carboxymethyl- dextran with hydrophobic anchor groups.CMDP, CMD50M, CMD50D
HC30M
Assays in serum or culture medium, positively charged analytes or contaminants.Hydrogel, 30 – 1000 nm, medium density. Linear polycarboxylate with reduced charge density.HLC30M, HLC200M, HLC1000M
Activation with sulfo-NHS required.
2D SPR micorarrays (spotting)NHS-preactivated hydrogel, >100 nm, linear polycarboxylate.HCX200M, HCX1000M
2D SPR micorarrays (flow printing)As standard SPR (see above).As standard SPR (see above).
Protein – peptide/small moleculeHydrogel, >500 nm, medium-high density. Linear polycarboxylate or carboxylated polysaccharideCMD500M
CMD700M
HC1000M, HC1500M
Vesicles and liposomes Lipophilic anchor groups attached to a CM dextran hydrogel. Captures whole vesicles and liposomes.LD
Supported lipid bilayers, Transmembane proteinsLipophilic anchor groups attached to a planar CM dextran surface. Substrate for lipid bilayer formation and vesicle spreading.LP
AntibodiesRecombinant Protein A/G derivatized CMD or HC polymer. It consists of 7 IgG-binding domains and its binding capacity is broader than either Protein A or Protein G alone. Protein A/G binds reversible and directed to all human IgG subclasses, IgA, IgE, IgM and to a lesser extent IgD; however, it does not bind mouse IgA, IgM or murine serum albumin.PAGD50, -200, -500 &-700L/M
PAGHC30, -200, -1000 & -1500M
PAGP
DNA, Proteins & peptides with low pIZwitterionic surface for covalent immobilization of negatively charged molecules via NHS chemistryZC30M
ZC80M
ZC150D
ZC150M
Difficult to couple proteins, peptides, small molecules, carbohydrates, nucleic acids. Surfaces derivatized with UV crosslinker. UV crosslinkers are extremely reactive upon irradiation with near UV (UV-A) and target many functional groups (-OH, -NH2 including less reactive C-H and C-C bonds).UVD50, -200,-500 & -700, UVDP
UVHC30, -200, -1000 & -1500
UVHCP
Click couplingDBCO modified linear polycarboxylate and carboxymethylated dextran sensor chips for immobilization of azide modified ligandsDCD50L, DCD200M

Letter codes represent different available hydrogel thicknesses and/or porosities. In the case of dextran-based hydrogels, 4 different thicknesses (20, 50, 200 and 700 nm) can be combined with 3 chain densities (low, medium and dense). The full product code would - for example - be SCBS CMD 200M, which stands for Sensor Chip Biacore™ series S compatible Carboxymethyldextran 200 nm, Medium density hydrogel. The standard functional group density of the modified dextrans is one per anhydroglucose unit. Lower degrees of functionalization, which can be useful to reduce non-specific interactions, are available as well.

XanTec’s product code nomenclature
XanTec’s product code nomenclature: Starting with the abbreviation for the SPR system used (red), the abbreviation for the polymer or polymer modification (blue) follows. This is combined with the hydrogel thickness (green), the hydrogel density (magenta), and the number of sensor chips per pack. (gray)

2D coatings

These planar coatings are less than 5 nm thick, allowing immobilization of single monolayers of small ligands, reaching densities of approximately 1 ng protein / mm². Their primary application is kinetic analysis, BIA of analytes with high molecular weight and capture of particulate analytes such as viruses, cells and particles.

2D coatings
Surface codeProduct description
AUBare gold. Not derivatized.
BPBiotin, covalently immobilized on a 2D saccharide monolayer.
CP     carboxyl functionalized self-assembled alkyl monolayer
CMDP2D carboxymethyldextran surface.
CMPGDendritic polyglycerol, carboxymethylated.
CMTEGTetraethylene glycol monolayer, carboxymethylated.
DP      2D dextran surface
HZPHydrazomodified 2D carboxyl surface.
HPPHydrophobic planar alkyl layer.
LP      2D carboxymethyldextran surface, partially alkyl derivatized
NAPNeutravidin, covalently immobilized on a 2D carboxymethyldextran surface.
NIPNi²+ ions complexed on a 2D chelating surface.
OHPDense 2D saccharide layer. Not modified.
PAPProtein A, covalently immobilized on a 2D carboxymethyldextran surface.
SAPStreptavidin, covalently immobilized on a 2D carboxymethyldextran surface.
TEGTetraethylene glycol monolayer.
TPDisulfide modified 2D carboxymethyldextran surface.

Hydrogel surfaces

3D immobilization matrices allow multilayer ligand immobilization which leads to significant signal amplification. They are employed for qualitative screening, concentration determinations and detection and kinetic analysis of low molecular weight compounds. As a rule of thumb, the smaller the analyte is, the thicker and denser the hydrogel structure should be.

The new hydrogel coatings of the HC family are currently available in four different thickness / porosity combinations: HC30M, HC200M, HC1000M and HC1500M. The HC type is based on a linear, synthetic polycarboxylate. Compared to the branched dextrans, it is better defined, shows a higher signal to noise ratio, has improved diffusion properties (better curve fits) and lower nonspecific interactions. Even more inert is the new HLC version which is available in the same thickness density combinations.

Hydrogel surfaces
Surface codeProduct description
AGAgarose hydrogel.
ALAlginate hydrogel.
AZD xAzide modified carboxymethyldextran surface. Click coupling.
50L, 200M
AZHC xAzide modified linear polycarboxylate hydrogel, medium charge density. Click coupling.
30M, 1500M
BDxBiotin, immobilized in a carboxymethyldextran hydrogel.
50L, 50M, 50D, 200L, 200M, 200D, 500L, 500M, 500D, 700L, 700M
BHC      Biotin, immobilized in a polycarboxylate hydrogel.
30M, 200M, 1000M, 1500M
C19RBDHC30M                                  Receptor Binding Domain (RBD) of SARS-CoV-2 Spike Protein immobilized on HC30M hydrogel
C xLinear polycarboxylate hydrogel, high charge density. Immobilization of nucleic acids.
30M, 80M, 150D
CMCCarboxymethylcellulose hydrogel.
CMD xxCarboxymethyldextran hydrogel.
50L, 50M, 50D, 200L, 200M, 200D, 500L, 500M, 500D, 700L, 700M
CMPG xCarboxymethylpolyethyleneglycol. Molecular weight: 4 kDa, alternative Mws upon request.
CS xxPolycarboxylate hydrogel, partially sulfonated. Preconcentration/immobilization of ligands with low pI.
30M, 80M, 150D
D xDextran hydrogel.
50M, 200M, 500M
DCD xDBCO modified carboxymethyldextran surface. Click coupling.

50L, 200M
GELGelatin hydrogel.
HC x Polycarboxylate hydrogel, medium charge density. Protein immobilization, standard.
30M, 200M, 1000M, 1500M
HEP Heparin. Approx. 50 nm hydrogel.
HLC Polycarboxylate hydrogel, low charge density. Extremely low level of non-specific interactions.
30M, 200M, 1000M, 1500M
HYHyaluronic acid hydrogel. Thin hydrogel with low non-specific interactions.
HZHC xHydrazide modified linear polycarboxylate hydrogel.
30M, 200M, 1000M, 1500M
LDLipophilic anchor groups attached to a 500 nm CM dextran hydrogel.
NAD xNeutravidin, immobilized in a carboxymethyldextran hydrogel. 

50L, 200M, 700L
NAHC xNeutravidin, immobilized in a linear polycarboxylate hydrogel. 

30M, 200M, 1500M
NIHC xPoly - NTA derivatized linear polycarboxylate hydrogel with high affinity.
30M, 200M, 1000M, 1500M
NID xxNTA derivatized carboxymethyldextran hydrogel.
50L, 50M, 50D, 200L, 200M, 200D, 500L, 500M, 500D, 700L, 700M
PAGDProtein A/G derivatized carboxymethyldextran hydrogel.
50L, 50M, 200L, 200M, 500L, 700L
PAGHCProtein A/G derivatized linear polycarboxylate hydrogel.
30M, 200M, 1000M
PCPectin hydrogel.
PEGPolyethyleneglycol. Molecular weight: 6 kDa, alternative MWs upon request.
PLY Poly-L-lysine.
SAHC xStreptavidin, immobilized in a linear polycarboxylate hydrogel.
30M, 200M, 1000M, 1500M
SAD xStreptavidin, immobilized in a carboxymethyldextran hydrogel.
50L, 50M, 50D, 200L, 200M, 200D, 500L, 500M, 700L, 700M
THC xDisulfide modified linear polycarboxylate hydrogel.
30M, 200M, 1000M, 1500M
TD xDisulfide modified carboxymethyldextran hydrogel.
50L, 50M, 50D, 200L, 200M, 200D, 500L, 500M, 500D
UVD UV crosslinker immobilized in a carboxymethyldextran hydrogel.
50L, 50M, 200L, 200M, 500L, 500M, 700L, 700M
ZC Zwitterionic hydrogel. Cationic after NHS activation.
30M, 80M
ZCC Cationic, zwitterionic hydrogel for pre-concentration scouting.
30M, 80M

The above mentioned SPR substrates are mainly intended for biomolecular interaction analysis, but may also be used as intermediate platforms for further modifications. The 2D and 3D coatings are covalently coupled to a protective interlayer and resist non-oxidative aqueous solutions from pH 1 - 13 as well as most organic solvents.

Alternative formats and custom coatings available upon request.