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2SPR

2-Channel Surface Plasmon Resonance System

The best 2-Channel SPR value you can buy.

Choose Reichert’s 2-channel surface plasmon resonance (SPR) system that lets you do more — and costs up to 30% less than the leading competitor. Our 2SPR System pushes the limits of detection and sensitivity, which is why it’s used by leading universities, academic facilities and biotech startups around the world for label-free biomolecular interaction analysis.

With the 2SPR, you get more, including:

• High-quality, publication-ready data
• The most sensitive, flexible and affordable 2-channel system available
• The best performance-to-cost ratio for individual labs
• A proven SPR leader since 1997

To see how our 2SPR System can help advance your research and lead to new breakthroughs, contact us today for further information or to schedule a demo.

Details

Save up to 30% with our 2-Channel SPR — the affordable choice

Spend less money on equipment and maintenance — and more on your research — when you choose a 2SPR. In fact, the typical research lab can save $17,000 per year, per system, compared to the equivalent performing systems. That’s $85,000 over the next five years — valuable funds that you can use for your staff and other critical research needs.

Lower equipment costs

Purchasing a 2SPR System typically costs $25,000 less than the leading competitor.

Lower operating costs

Every SPR machine requires chips to couple proteins to. A typical lab can use 75–200 sensor chips each year, which is why you should always ask about the cost of chips when you’re considering an SPR system. XanTec bioanalytics offers the biggest choice of high-quality sensor chips plus reagents which cost significantly less than our competitors, so you can run more experiments without worrying about out-of-control costs.

No costly service contract required

Other SPR companies make you purchase a service contract, which can easily cost up to $150,000 over the course of five years. With our 2SPR System, all of the typical parts that need maintenance are easy for you to access (and service) yourself. Of course, we do offer service contracts, if you would rather let us handle the regular maintenance.

Open system = high-quality data, more flexibility

Get high-quality, publication-ready data

The Reichert 2SPR System offers significant advantages for researchers and scientists. Whether you’re working with simple solutions or more challenging assays, you can count on Reichert to offer:

• High sensitivity — study protein interactions with low molecular weight (down to approximately 100Da) compounds; enhanced sensitivity also makes the 2SPR System ideal for:
  • High quality kinetic analysis
  • Affinity measurements for weak or strong interactions (ranging from 1mM to 1pM)
  • Precise determination of thermodynamic parameters
  • Accurate concentration analysis
• Industry-leading low drift (0.1 μRIU/min)
• Extremely low noise (0.05 μRIU rms)
• Faster baseline stability — the Reichert2SPR System stabilizes 2x-3x faster than the competition, due to our streamlined fluidics
• High sample capacity (up to 768 samples per run)

Open system = more control, more flexibility, more time for research

Other SPR systems only allow for limited access to critical parts. But with the 2SPR, everything you need to access is right out in the open, which makes your life — and your research — much easier.

Easy to change as your research evolves

The Reichert 2SPR System is designed to be as flexible as possible, from changeable tubes to an easily accessible flow cell. Wherever your research takes you in the future, you can be rest assured that we will let you stay on track, without costly upgrades and changes.

5-minute tubing changes

With our SPR systems, you can change the tubing whenever you want in just minutes — without having to wait around for a service call. That’s because all Reichert SPR systems are designed to use low-cost, off-the-shelf HPLC fittings and tubing that are easily accessible to you and your staff. With these systems, you can:

• Change the tubing to different sizes
• Accommodate a wide range of sample compositions (from pure soluble solutions to cell lysates), varying viscosities, and particulate matter
• Run crude samples without having to purify them — saving you time and providing even greater flexibility — without risking costly delays and service calls; crude samples include:
  • Whole cells
  • Viruses
  • Lysates
  • Serum
  • Aggregates
• Stop worrying about clogs — if a tube is damaged, blocked or clogged, you can replace it yourself in just a few minutes, using standard tubing

Easy-to-clean flow cells

While some competitors require you to replace a flowcell cartridge once or twice a year, our flow cells are easy to clean (and, if necessary, change) yourself and are not a consumable. We offer standard flow cells, as well as specialized flow cells that can be used simultaneously with other valuable analytical techniques, opening up new opportunities for investigation in SPR experiments.

Widest variety of standard and specialized chips

Our sensor chips perform equivalently or better to the leading competitor’s chips, at a significantly lower cost. Additionally to the standard chips, XanTec offers several unique functionalities, found nowhere else on the market. These include:

• Carboxymethyl dextra
• Linear polycarboxylate with extremely low background
• Zwitterionic hydrogels
• Standard and high-affinity Nickel nitrilotriacetic acid
• Azide and DBCO derivatives for click chemistry
• Streptavidin / NeutrAvidin
• Protein A / Protein A/G
• Strep-Tactin XT
• Spycatcher
• Plain gold (if you want to add your own chemistry)

Interface with other modules for all-in-one experimentation options

Reichert’s open, component-based design lets you connect our 2SPR System to other modules, and gain valuable new information in a single experiment.

Our specialized flow cells let you:

• Combine our SPR systems with:
  
  • Electrochemistry
  • Fluorescence
  • Photochemistry
• Couple our SPR systems with:
  
  • Mass spectrometry
  • Liquid chromatography

About

XanTec and Reichert – a powerful team since 25 years

XanTec and Reichert are names you can trust. Both have been developing cutting-edge SPR technology since 1997, selling to some of the top names in academics and private industry. While the instrument manufacturer Reichert has been in business for more than 140 years and is backed by the billion-dollar company, AMETEK, Inc., XanTec is offering the biggest sensor chip portfolio available on the market today.

Personal service and attention, whenever you need it

At XanTec, we’re here for you — just as we have been for more than 25 years — with the expert service and support you expect from an industry leader.

• Product demo: We offer instrument demonstrations either in our or in your lab, show you how to run your samples so you can see the results for yourself. Request a demonstration.
• In-person set-up and training: When you purchase one of our SPR systems, we’ll come to your lab to set everything up for you and help train your staff.
• Ongoing support and troubleshooting: If you ever have a question or problem, just give us a call or send an email. We’ll help you address any issues, at any time.

Surface plasmon resonance (SPR) is a technique that lets you investigate biomolecular interactions (including, but not limited to, protein-protein binding and protein-small molecule binding) in real-time. With SPR, you can determine if there is an interaction with your protein or other target molecule — and, if so — answer questions about the interaction including:

• How fast do the molecules associate and dissociate?
• How strong is the interaction (affinity)?
• How specific is it?

If you have questions about whether or not SPR is right for your lab, please contact us.

For early-stage life sciences and material science research

SPR is typically used in academic and industry settings to advance research in a range of areas, including:

• Antibody development
• Drug development and discovery
• Concentration analysis
• Gene regulation
• Immunogenicity
• Nanoparticles/Nanomaterials
• Pharmaceutical research
• Studying protein structure and function determination
• Systems biology
• Thermodynamics analysis (ΔH & ΔS)

Whether you’re studying Alzheimer’s disease, developing new vaccines or focused on other areas of protein research, SPR can help you quickly determine whether proteins interact, and which ones are worth studying more.

Label-free technique saves you time

With SPR, you work with native proteins, which means you don’t have to attach another molecule to one of your proteins. Instead, you’ll use our SPR device to immobilize one of your proteins on a small “chip,” and then flow your other protein or molecule over the chip to see the response and study the interaction.

Determine the binding properties of bimolecular interactions

SPR allows you to study a variety of interactions, including those involving:

• Proteins
• Nucleic acids
• Lipids
• Carbohydrates
• Small molecules
• Bacteria
• Polymers

With alternative tubing, you can also perform SPR on crude samples, including:

• Whole cells
• Viruses
• Lysates
• Serum

SPR: More data, greater accuracy than other techniques

With SPR, you get a large volume of highly accurate information about protein interactions, including the strength of the interactions (affinities) and the binding kinetics — how fast the molecules are binding (associating) and dissociating. Other techniques don’t provide as much data and aren’t as accurate.

Specs/Compare

Technical information

Measurement sensitivity

Typical kinetic and equilibrium constant ranges

Compare our 2SPR to Cytiva Biacore™ X100

The Reichert2SPR - SR7500DC system is the smart choice for scientists and lab managers. Our surface plasmon resonance instruments provide a highly sensitive and flexible platform to meet the demands of a variety of applications. Reichert’s low maintenance costs, autosampler capacity, and sensitivity make it the obvious choice.

Software

2SPR system software

Reichert's powerful software platform for all 2-channel instruments, provides integrated data acquisition, automated sample handling, and data analysis:

• Integrated sophisticated control of the 2SPR System
• Flexible, intuitive, step-by-step control of the Surface Plasmon Resonance system.
• Drag and drop methods to set up multi-sample run tables.
• Manual control of each component individually without constraints – the ultimate flexibility for real-time method development

Our SPR software is validated with 21 CFR part 11 technical controls

Protecting the security and integrity of electronic records (ER) is essential for compliance. This includes ensuring the reliability and trustworthiness of ER used to support critical decisions. Features in Reichert’s SPR software include:

• Data security and integrity – access control along with file encrypted checksums
• User authorization levels – administrator, factory, and user levels set access rights to software functions
• Record tracking – experiment details, events, and user identities are logged in a date- and timed-stamped secure file
• Data can be exported both manually and automatically in a variety of formats, including tab delineated text files and Scrubber® data analysis files

The software has been developed in accordance with an accepted development model to ensure adequate validation.

Experimental method development

B program run table screen

Developing an experimental method with drag and drop functionality is easy. To begin, a standard method can be dragged and dropped from the “Available Methods” section (center) to the run table window. The method can be quickly modified as necessary by the researcher. User defined methods can be saved for reuse in future experiments. In the example sequence, step 1 is a pump refill and step 2 is a 1-minute wait for baseline stabilization. The third step is a 2 minute 100µL sample injection from vial 1A followed by a 5 minute dissociation period. The final steps are a 50µL injection of a regeneration solution followed by a 1 minute dissociation. The checked “Rinse” box in an injection method washes the sample loop and needle with buffer prior to the next sample injection. Multiple injections are easily programmed by simply dragging and dropping a “Loop” command at the beginning and end of the methods that are to be run several times. The number of loops is set in a user-definable box in the loop end command. The vial position will automatically increment. This functionality easily facilitates scale-up of experiments in minutes, to take advantage of Reichert's high capacity autosampler - including injections from 96 and 384 well plates or 48 or 12 vial sample trays.

Data acquisition window

Data acquistion-real time visualization

Data from the individual channels and difference can be viewed together or separately in real-time. Both the response (Y axis) and time (X axis) axis may be auto-scaled or set to user-defined limits. Individual cycles can be selected to quickly zoom in on an area of interest. The data window can also be expanded over a defined region to allow close examination of the SPR response curves. In addition, this window continuously displays the status of each system component, read time interval, flow rate, temperature, file name and injection/dissociation markers.

Post process window

Post process

SPR sensorgrams are automatically extracted and aligned to the same zero start time and zero response. The aligned sensorgrams are then sent to the analysis package. In addition to Reichert's integrated package, data can be exported for use in other widely available packages, such as Scrubber.

Analysis

Aligned sensorgrams are automatically transferred to the integrated data analysis package in one click. Once in the analysis module, users can fine-tune the alignment, perform background and blank subtractions, and adjust concentrations, if needed. Creating kinetic fits is a straight forward task, and results are clearly displayed. There are a number of kinetics models to choose from to ensure appropriate characterization of the experimental data. Data can be fit to a 1:1 binding model (with or without mass transport or depletion), or three different 2-site models (2:1 with or without conformational change, or 1:2). You can also fit Dissociation data only. Figure 1 shows results for data fit to a 1:1 binding model.

Additionally, when equilibrium responses are obtained, affinity analyses can be completed to create figures that are publication- ready (see Figure 2).

Summaries of ka(association rate constant) an kd(dissociation rate constant) can be presented as on/off plots by clicking the On/off rate map button in the summary tool. Each curve is presented as a coordinate in the ka/kd-space, which enables a fast overview of many compounds.

Reichert's Surface Plasmon Resonance software provides flexibility for users, enabling full control of experimental methods easily transition from collection to analysis to answers.

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