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Products | SPR Biosensors | SPR Systems | 4SPR

 

4SPR

4-Channel Surface Plasmon Resonance System

The more flexible, more powerful, more affordable 4-Channel SPR.

With Reichert’s 4SPR, you get a four-channel surface plasmon resonance (SPR) system that provides outstanding data, with a 30-70% lower total cost of ownership over five years as compared to equivalent performing systems.

The 4SPR gives you:

 

To see how our 4SPR can help advance your research and lead to new breakthroughs, contact us today to schedule a demo.

4SPR 4SPR 4SPR

Details

Save Up To 40% with our 4-Channel SPR — the affordable choice

Spend less money on equipment and maintenance — and more on your research — when you choose one of our 4SPR systems. In fact, the typical research lab can save $25,000 per year. That’s $125,000 over the next five years — valuable funds that you can use for your staff and other critical research needs.

Lower equipment costs

Less initial capital expense means more money for you to address other laboratory needs.

Lower operating costs

Every SPR machine requires a sensor chip to attach proteins to. A typical lab can use 75–200 sensor chips each year, which is why you should always ask about the cost of chips when you’re considering an SPR system. XanTec bioanalytics offers the biggest choice of high-quality sensor chips plus reagents which cost significantly less than our competitors, so you can run more experiments without worrying about out-of-control costs.

No costly service contract required

Other SPR companies make you purchase a service contract, which can easily cost up to $150,000 over the course of five years. With our 4SPR System, all of the typical parts that need maintenance are easy for you to access (and service) yourself. In fact, the amount you’ll save on maintenance over five years is nearly the amount you would spend on acquiring a brand new instrument. Of course, we do offer service contracts, if you would rather let us handle the regular maintenance.

Open system = high-quality data, more flexibility

GET HIGH-QUALITY, PUBLICATION-READY DATA
The Reichert4SPR System offers significant advantages for researchers and scientists. Whether you’re working with simple solutions or more challenging assays, you can count on our systems to offer:

Open system = more control, more flexibility, more time for research

Other SPR systems only allow for limited access to critical parts. But with the 4SPR, everything you need to access is right out in the open, which makes your life — and your research — much easier.

Easy to change as your research evolves

The Reichert 4SPR System is designed to be as flexible as possible, from changeable tubes to an easily accessible flow cell. Wherever your research takes you in the future, you can be rest assured that Reichert will let you stay on track, without costly upgrades and changes.

5-minute tubing changes

With our SPR systems, you can change the tubing whenever you want in just minutes — without having to wait around for a service call. That’s because all Reichert SPR systems are designed to use low-cost, off-the-shelf HPLC fittings and tubing that are easily accessible to you and your staff. With these systems, you can:

Easy-to-clean flow cells

While some competitors require you to you to replace a flow cell cartridge once or twice a year, our flow cells are easy to clean (and, if necessary, change) yourself and are not a consumable. We offer standard flow cells, as well as specialized flow cells that can be used simultaneously with other valuable analytical techniques, opening up new opportunities for investigation in SPR experiments.

A wide variety of standard and specialized chips

Our sensor chips perform equivalently or better to the leading competitor’s chips, at a significantly lower cost. Additionally to the standard chips, XanTec offers several unique functionalities, found nowhere else on the market. These include:

Four channels for greater efficiency and throughput

The Reichert4SPR System is a four-channel system, which means you can run up to three experiments at one time. Speed up your method development by testing different immobilization chemistries or regeneration schemes on each channel.

With four channels, you have the flexibility to run:

We also sell a two-channel and a three-channel system for labs that don’t require the throughput of a 4-channel instrument.

About

XanTec and Reichert – a powerful team since 25 years

XanTec and Reichert are names you can trust. Both have been developing cutting-edge SPR technology since 1997, selling to some of the top names in academics and private industry. While the instrument manufacturer Reichert has been in business for more than 140 years and is backed by the billion-dollar company, AMETEK, Inc., XanTec is offering the biggest sensor chip portfolio available on the market today.

Personal service and attention, whenever you need it

At XanTec, we’re here for you — just as we have been for more than 25 years — with the expert service and support you expect from an industry leader.

Surface plasmon resonance (SPR) is a technique that lets you investigate biomolecular interactions (including, but not limited to, protein-protein binding and protein-small molecule binding) in real-time. With SPR, you can determine if there is an interaction with your protein or other target molecule — and, if so — answer questions about the interaction including:

If you have questions about whether or not SPR is right for your lab, please contact us.

For early-stage life sciences and material science research

SPR is typically used in academic and industry settings to advance research in a range of areas, including:

Whether you’re studying Alzheimer’s disease, developing new vaccines or focused on other areas of protein research, SPR can help you quickly determine whether proteins interact, and which ones are worth studying more.

Label-free technique saves you time

With SPR, you work with native proteins, which means you don’t have to attach another molecule to one of your proteins. Instead, you’ll use our SPR device to immobilize one of your proteins on a small “chip,” and then flow your other protein or molecule over the chip to see the response and study the interaction.

Determine the binding properties of bimolecular interactions

SPR allows you to study a variety of interactions, including those involving:

With alternative tubing, you can also perform SPR on crude samples, including:

SPR: More data, greater accuracy than other techniques

With SPR, you get a large volume of highly accurate information about protein interactions, including the strength of the interactions (affinities) and the binding kinetics — how fast the molecules are binding (associating) and dissociating. Other techniques don’t provide as much data and aren’t as accurate.

Specs/Compare

Technical information

Measurement Channels Four
Sample Loading Autosampler. Up to 768 samples
Injection Volume 1 uL to 4,500 uL (depends on installed loop volume)
Buffer Degasser Built-in
Temperature Range 10°C below ambient to 70°C
Sample Storage 4°C or ambient temperature
21 CFR Part 11 Compliant

Measurement sensitivity

Baseline Noise 0.05 uRIU (RMS)
Baseline Drift < 0.1 uRIU/min
Minimum Molecular Weight Detection No lower limit for organic molecules

Typical kinetic and equilibrium constant ranges

Association Rate Constant 103to 108M-1s-1
Dissociation Rate Constant 10-1to 10-6s-1
Equilibrium Dissociation Constant 10-3to 10-12M

Compare our 4SPR to Cytiva Biacore™ T200

The Reichert4SPR, is the new standard for Surface Plasmon Resonance (SPR) instrumentation. Our 4-Channel system features high sensitivity with maximum flexibility and uptime to meet even your most demanding applications. The Reichert4SPR provides more value by featuring easy-to-access fluidics, lower operating and maintenance costs, and maximum flexibility with no compromise in performance.

Reichert4SPR Cytiva Biacore™ T200
Number of Channels 4 4
DIY Fluidics Yes No
Autosampler Capacity Maximum 768 samples Maximum 462 samples
Baseline Drift 0.1 RU/min < 0.3 RU/min
Baseline Noise 0.05 RU(RMS) 0.03 RU(RMS)
Specialized Flow Cells Electrochemistry, Photochemistry,
Fluorescence, Mass Spectrometry
None
Temperature Range 10°C below ambient to 70°C 4 - 45°C
Flow Rate Range 0.1 - 3000 µL/min 1 – 100 µL/min
Minimum Molecular Weight Detection No lower limit for organic molecules No lower limit for organic molecules

Software

Reichert sets the standard for easy-to-use SPR with new software for the 4SPR - 4-Channel SPR Instrument. Redesigned with the scientist in mind, the new software is intuitive, easy-to-use and powerful.

SPR software opening screen

Listening to feedback from scientists who were frustrated with other SPR systems that made them feel like a computer programmer, Reichert’s new software is designed around the scientist and their needs. This new instrument control software guides the user through the natural workflow of an experiment – from immobilization to method development and through scale up of large screening runs. No training is required and the concept of “programming” a run does not exist – allowing the focus to be on the science, while the software runs the instrument. The new software significantly reduces experiment set-up time and manual entry for the scientist. The enhanced 4SPR is intuitive and only asks the scientist about relevant experimental details.

SPR software start screen

An important benefit of our software is the organization of projects and experiments. The system does it for the user. Want to build on a previous experiment? Call it up, modify only the parameters they want to change and begin. The software is perfect for core facilities and pharma/biotech companies. With a welcoming, clean look and feel, the software is not intimidating and easily walks you through the set up and execution of your experiment.

SPR software scale up screen

Consistent with Reichert’s philosophy, the software is ready to support GxP environments and is 21 CFR Part 11 compliant. No additional software or modules are required. Existing 4SPR systems are compatible with the new software and the upgrade is available. With the new software, we take ease of use to an entirely new level, while maintaining the extremely high sensitivity and flexibility scientists require for the most demanding and creative experiments.

Figure 1
SPR software opening screen
Figure 2
SPR software start screen
Scale Up Screen
SPR software scale up screen

Analysis

Aligned sensorgrams are automatically transferred to the integrated data analysis package in one click. Once in the analysis module, users can fine-tune the alignment, perform background and blank subtractions, and adjust concentrations, if needed. Creating kinetic fits is a straight forward task, and results are clearly displayed. There are a number of kinetics models to choose from to ensure appropriate characterization of the experimental data. Data can be fit to a 1:1 binding model (with or without mass transport or depletion), or three different 2-site models (2:1 with or without conformational change, or 1:2). You can also fit Dissociation data only. Figure 1 shows results for data fit to a 1:1 binding model.

Additionally, when equilibrium responses are obtained, affinity analyses can be completed to create figures that are publication- ready (see Figure 2).

Summaries of ka(association rate constant) an kd(dissociation rate constant) can be presented as on/off plots by clicking the On/off rate map button in the summary tool. Each curve is presented as a coordinate in the ka/kd-space, which enables a fast overview of many compounds.

Reichert's Surface Plasmon Resonance software provides flexibility for users, enabling full control of experimental methods easily transition from collection to analysis to answers.

Figure 1
Figure 1: Data fit to a 1:1 Binding Model
Figure 2
Figure 2: Sample Affinity Plot
Figure 3
Figure 3: Easily create publication-ready figures, such as this summary of screening of five different compounds over the same surface.

Brochures

Title Document Download
COMPARISON 7500-T200-X100 REVISED OCTOBER 2015 7500-T200-X100  Download
FINAL-REICHERT-4-CHANNEL-SPR-SYSTEM-9-16 FINAL-REICHERT-4-CHANNEL-SPR-SYSTEM-9-16 Download