With Reichert’s 4SPR, you get a four-channel surface plasmon resonance (SPR) system that provides outstanding data, with a 30-70% lower total cost of ownership over five years as compared to equivalent performing systems.
To see how our 4SPR can help advance your research and lead to new breakthroughs, contact us today to schedule a demo.
Spend less money on equipment and maintenance — and more on your research — when you choose one of our 4SPR systems. In fact, the typical research lab can save $25,000 per year. That’s $125,000 over the next five years — valuable funds that you can use for your staff and other critical research needs.
Less initial capital expense means more money for you to address other laboratory needs.
Every SPR machine requires a sensor chip to attach proteins to. A typical lab can use 75–200 sensor chips each year, which is why you should always ask about the cost of chips when you’re considering an SPR system. XanTec bioanalytics offers the biggest choice of high-quality sensor chips plus reagents which cost significantly less than our competitors, so you can run more experiments without worrying about out-of-control costs.
Other SPR companies make you purchase a service contract, which can easily cost up to $150,000 over the course of five years. With our 4SPR System, all of the typical parts that need maintenance are easy for you to access (and service) yourself. In fact, the amount you’ll save on maintenance over five years is nearly the amount you would spend on acquiring a brand new instrument. Of course, we do offer service contracts, if you would rather let us handle the regular maintenance.
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The Reichert4SPR System offers significant advantages for researchers and scientists. Whether you’re working with simple solutions or more challenging assays, you can count on our systems to offer:
Other SPR systems only allow for limited access to critical parts. But with the 4SPR, everything you need to access is right out in the open, which makes your life — and your research — much easier.
The Reichert 4SPR System is designed to be as flexible as possible, from changeable tubes to an easily accessible flow cell. Wherever your research takes you in the future, you can be rest assured that Reichert will let you stay on track, without costly upgrades and changes.
With our SPR systems, you can change the tubing whenever you want in just minutes — without having to wait around for a service call. That’s because all Reichert SPR systems are designed to use low-cost, off-the-shelf HPLC fittings and tubing that are easily accessible to you and your staff. With these systems, you can:
While some competitors require you to you to replace a flow cell cartridge once or twice a year, our flow cells are easy to clean (and, if necessary, change) yourself and are not a consumable. We offer standard flow cells, as well as specialized flow cells that can be used simultaneously with other valuable analytical techniques, opening up new opportunities for investigation in SPR experiments.
Our sensor chips perform equivalently or better to the leading competitor’s chips, at a significantly lower cost. Additionally to the standard chips, XanTec offers several unique functionalities, found nowhere else on the market. These include:
The Reichert4SPR System is a four-channel system, which means you can run up to three experiments at one time. Speed up your method development by testing different immobilization chemistries or regeneration schemes on each channel.
With four channels, you have the flexibility to run:
We also sell a two-channel and a three-channel system for labs that don’t require the throughput of a 4-channel instrument.
XanTec and Reichert are names you can trust. Both have been developing cutting-edge SPR technology since 1997, selling to some of the top names in academics and private industry. While the instrument manufacturer Reichert has been in business for more than 140 years and is backed by the billion-dollar company, AMETEK, Inc., XanTec is offering the biggest sensor chip portfolio available on the market today.
At XanTec, we’re here for you — just as we have been for more than 25 years — with the expert service and support you expect from an industry leader.
Surface plasmon resonance (SPR) is a technique that lets you investigate biomolecular interactions (including, but not limited to, protein-protein binding and protein-small molecule binding) in real-time. With SPR, you can determine if there is an interaction with your protein or other target molecule — and, if so — answer questions about the interaction including:
If you have questions about whether or not SPR is right for your lab, please contact us.
SPR is typically used in academic and industry settings to advance research in a range of areas, including:
Whether you’re studying Alzheimer’s disease, developing new vaccines or focused on other areas of protein research, SPR can help you quickly determine whether proteins interact, and which ones are worth studying more.
With SPR, you work with native proteins, which means you don’t have to attach another molecule to one of your proteins. Instead, you’ll use our SPR device to immobilize one of your proteins on a small “chip,” and then flow your other protein or molecule over the chip to see the response and study the interaction.
SPR allows you to study a variety of interactions, including those involving:
With alternative tubing, you can also perform SPR on crude samples, including:
With SPR, you get a large volume of highly accurate information about protein interactions, including the strength of the interactions (affinities) and the binding kinetics — how fast the molecules are binding (associating) and dissociating. Other techniques don’t provide as much data and aren’t as accurate.
Measurement Channels | Four |
Sample Loading | Autosampler. Up to 768 samples |
Injection Volume | 1 uL to 4,500 uL (depends on installed loop volume) |
Buffer Degasser | Built-in |
Temperature Range | 10°C below ambient to 70°C |
Sample Storage | 4°C or ambient temperature |
21 CFR Part 11 | Compliant |
Baseline Noise | 0.05 uRIU (RMS) |
Baseline Drift | < 0.1 uRIU/min |
Minimum Molecular Weight Detection | No lower limit for organic molecules |
Association Rate Constant | 103to 108M-1s-1 |
Dissociation Rate Constant | 10-1to 10-6s-1 |
Equilibrium Dissociation Constant | 10-3to 10-12M |
The Reichert4SPR, is the new standard for Surface Plasmon Resonance (SPR) instrumentation. Our 4-Channel system features high sensitivity with maximum flexibility and uptime to meet even your most demanding applications. The Reichert4SPR provides more value by featuring easy-to-access fluidics, lower operating and maintenance costs, and maximum flexibility with no compromise in performance.
Reichert4SPR | Cytiva Biacore™ T200 | |
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Number of Channels | 4 | 4 |
DIY Fluidics | Yes | No |
Autosampler Capacity | Maximum 768 samples | Maximum 462 samples |
Baseline Drift | 0.1 RU/min | < 0.3 RU/min |
Baseline Noise | 0.05 RU(RMS) | 0.03 RU(RMS) |
Specialized Flow Cells | Electrochemistry, Photochemistry, Fluorescence, Mass Spectrometry |
None |
Temperature Range | 10°C below ambient to 70°C | 4 - 45°C |
Flow Rate Range | 0.1 - 3000 µL/min | 1 – 100 µL/min |
Minimum Molecular Weight Detection | No lower limit for organic molecules | No lower limit for organic molecules |
Reichert sets the standard for easy-to-use SPR with new software for the 4SPR - 4-Channel SPR Instrument. Redesigned with the scientist in mind, the new software is intuitive, easy-to-use and powerful.
Listening to feedback from scientists who were frustrated with other SPR systems that made them feel like a computer programmer, Reichert’s new software is designed around the scientist and their needs. This new instrument control software guides the user through the natural workflow of an experiment – from immobilization to method development and through scale up of large screening runs. No training is required and the concept of “programming” a run does not exist – allowing the focus to be on the science, while the software runs the instrument. The new software significantly reduces experiment set-up time and manual entry for the scientist. The enhanced 4SPR is intuitive and only asks the scientist about relevant experimental details.
An important benefit of our software is the organization of projects and experiments. The system does it for the user. Want to build on a previous experiment? Call it up, modify only the parameters they want to change and begin. The software is perfect for core facilities and pharma/biotech companies. With a welcoming, clean look and feel, the software is not intimidating and easily walks you through the set up and execution of your experiment.
Consistent with Reichert’s philosophy, the software is ready to support GxP environments and is 21 CFR Part 11 compliant. No additional software or modules are required. Existing 4SPR systems are compatible with the new software and the upgrade is available. With the new software, we take ease of use to an entirely new level, while maintaining the extremely high sensitivity and flexibility scientists require for the most demanding and creative experiments.
Aligned sensorgrams are automatically transferred to the integrated data analysis package in one click. Once in the analysis module, users can fine-tune the alignment, perform background and blank subtractions, and adjust concentrations, if needed. Creating kinetic fits is a straight forward task, and results are clearly displayed. There are a number of kinetics models to choose from to ensure appropriate characterization of the experimental data. Data can be fit to a 1:1 binding model (with or without mass transport or depletion), or three different 2-site models (2:1 with or without conformational change, or 1:2). You can also fit Dissociation data only. Figure 1 shows results for data fit to a 1:1 binding model.
Additionally, when equilibrium responses are obtained, affinity analyses can be completed to create figures that are publication- ready (see Figure 2).
Summaries of ka(association rate constant) an kd(dissociation rate constant) can be presented as on/off plots by clicking the On/off rate map button in the summary tool. Each curve is presented as a coordinate in the ka/kd-space, which enables a fast overview of many compounds.
Reichert's Surface Plasmon Resonance software provides flexibility for users, enabling full control of experimental methods easily transition from collection to analysis to answers.
Title | Document | Download |
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COMPARISON 7500-T200-X100 REVISED OCTOBER 2015 | 7500-T200-X100 | Download |
FINAL-REICHERT-4-CHANNEL-SPR-SYSTEM-9-16 | FINAL-REICHERT-4-CHANNEL-SPR-SYSTEM-9-16 | Download |