Products | SPR Sensor chips | Chip selection guide

Chip selection guide

To ensure optimal results, XanTec offers an extensive range of different sensor chips spanning a variety of polymers, densities, thicknesses and capture molecules. The following charts may serve as orientation to find the optimal chip surface dependent on assay format, type of interaction and interacting (bio-) molecules.

Suggested hydrogel thickness by molecular weight of analyte
Molecular weight of Analyte (Da) <100 100 – 1000 1k-10k 10k-150k >150k
Recommended hydrogel thickness (nm) ≥500 500 - 200 200 - 50 50 - planar
Suggested hydrogel density by molecular weight of analyte
Molecular weight of Analyte (Da) <100 100 – 1000 1k-10k 10k-150k >150k
Recommended hydrogel density dense dense-medium medium medium-low low
Polymer 01a
Application (ligand – analyte)Suggested structure / polymer typeSuitable sensor chip(s)
Protein – protein, assayHydrogel, 100 – 500 nm, low - medium density. Linear polycarboxylate or carboxymethyldextran.CMD200M, CMD500L, HC200M, (HC1000M)
Protein – protein, kinetics2D surface or thin hydrogel with low density.CMDP, CMD50L, CMD50M, CMD200L, HC30M
Protein – DNA or polysaccharideHydrogel, 30 – 1000 nm, medium density. Linear polycarboxylate.HC30M, HC200M, HC1000M
Protein – peptide/small moleculeHydrogel, >500 nm, high density. Linear polycarboxylate or carboxylated polysaccharideCMD500M
HC1000M, HC1500M
Protein – virus, particle or bacteria (cells)Thin (50 nm) hydrogel, 2D surface, medium-high density.  Linear polycarboxylate or carboxymethyldextran.CMDP, CMD50M
DNA – DNA, small molecules, peptidesHydrogel, > 200 nm, medium - high density. Streptavidin modified linear polycarboxylate or carboxymethyldextran.SAD500M, SAHC1000M
DNA – proteinHydrogel, 100 – 500 nm, low - medium density. Streptavidin modified carboxymethyldextran or linear polycarboxylate.SAD50M, SAD200L, SAD200M, SAD500L
DNA – proteinHydrogel, 30 – 500 nm, low - medium density. Neutravidin derivatized carboxymethyldextran or linear polycarboxylate. May reduced non-specific binding for positively charged analytes.NAD50M, NAD200M, NAD500L
Click coupling
Especially Sensitive Ligands (e.g. Proteins with restrictions in buffer composition) and all other proteins
Azide modified linear polycarboxylate and carboxymethylated dextran with DBCO modified ligand – DBCO Labeling Kit
Preferable alternative to biotin/streptavidin
Cell, virus, particle, lipid bilayers – any species2D surface or thin (50 nm) hydrogel, medium-high density.  Linear polycarboxylate or carboxymethyl- dextran with hydrophobic anchor groups.CMDP, CMD50M, CMD50D
Assays in serum or culture medium, positively charged analytes or contaminants.Hydrogel, 30 – 1000 nm, medium density. Linear polycarboxylate with reduced charge density.HLC30M, HLC200M, HLC1000M
Activation with sulfo-NHS required.
2D SPR micorarrays (spotting)NHS-preactivated hydrogel, >100 nm, linear polycarboxylate.HCX200M, HCX1000M
2D SPR micorarrays (flow printing)As standard SPR (see above).As standard SPR (see above).
Protein – peptide/small moleculeHydrogel, >500 nm, medium-high density. Linear polycarboxylate or carboxylated polysaccharideCMD500M

HC1000M, HC1500M
Vesicles and liposomes Lipophilic anchor groups attached to a CM dextran hydrogel. Captures whole vesicles and liposomes.LD
Supported lipid bilayers, Transmembane proteinsLipophilic anchor groups attached to a planar CM dextran surface. Substrate for lipid bilayer formation and vesicle spreading.LP
AntibodiesRecombinant Protein A/G derivatized CMD or HC polymer. It consists of 7 IgG-binding domains and its binding capacity is broader than either Protein A or Protein G alone. Protein A/G binds reversible and directed to all human IgG subclasses, IgA, IgE, IgM and to a lesser extent IgD; however, it does not bind mouse IgA, IgM or murine serum albumin.PAGD50, -200, -500 &-700L/M
PAGHC30, -200, -1000 & -1500M
DNA, Proteins & peptides with low pIZwitterionic surface for covalent immobilization of negatively charged molecules via NHS chemistryZC30M
Difficult to couple proteins, peptides, small molecules, carbohydrates, nucleic acids. Surfaces derivatized with UV crosslinker. UV crosslinkers are extremely reactive upon irradiation with near UV (UV-A) and target many functional groups (-OH, -NH2 including less reactive C-H and C-C bonds).UVD50, -200,-500 & -700, UVDP
UVHC30, -200, -1000 & -1500
Click couplingDBCO modified linear polycarboxylate and carboxymethylated dextran sensor chips for immobilization of azide modified ligandsDCD50L, DCD200M

Letter codes represent different available hydrogel thicknesses and/or porosities. In the case of dextran-based hydrogels, 4 different thicknesses (20, 50, 200 and 700 nm) can be combined with 3 chain densities (low, medium and dense). The full product code would - for example - be SCBS CMD 200M, which stands for Sensor Chip Biacore™ series S compatible Carboxymethyldextran 200 nm, Medium density hydrogel. The standard functional group density of the modified dextrans is one per anhydroglucose unit. Lower degrees of functionalization, which can be useful to reduce non-specific interactions, are available as well.

XanTec’s product code nomenclature
XanTec’s product code nomenclature: Starting with the abbreviation for the SPR system used (red), the abbreviation for the polymer or polymer modification (blue) follows. This is combined with the hydrogel thickness (green), the hydrogel density (magenta), and the number of sensor chips per pack. (gray)

Biacore™ users can find the XanTec sensor chip corresponding to their Biacore™ sensor chip in the following table.

Plain gold surfaceAuAU
No hydrogel, low capacityC1CMDP / HCP
Short matrix, normal capacity CM3CMD50L, HC30M
Standard hydrogel, low charge densityCM4HLC200M
Standard hydrogel, normal capacityCM5CMD200M
Standard hydrogel, high capacityCM7CMD700M, HC1500M
NTA for His6 tagged ligandsNTAHigh affinity: NiHC1500M, NiHC200M
Standard affinity: NiD200M
Hydrophobic 2D surfaceHPAHPP
Vesicle and liposome captureL1LD (hydrogel) & LP (2D)
Biotin capturing surfaceStreptavidin: SAD200M & others
Neutravidin: NAHC200M & others
Click chemistryAzide surface: AZHC200M & others
DBCO surface: DCD200M & others
Zwitterionic surfaceZC30M, ZC80M, ZC150D
Biotin derivatized surfaceBHC30M, BD200M & others