SARS-CoV-2 is a member of a large family of viruses called coronaviruses that causes a highly contagious respiratory disease called COVID-19. This has created a world-wide public-health emergency, affecting billions of lives. The development of therapeutics and vaccines to prevent the spread of SARS-CoV-2 is an area of enormous focus for scientists worldwide. Toward this end, researchers at Sichuan University and the XtalPi AI Research Center implemented an in-silico method called Rosetta Flex ddG to predict the binding affinity changes caused by point mutations on the receptor binding domain (RBD) of the SARS-CoV- 2 virus S (spike) protein. Their aim was to determine higher affinity mutants which could become more infectious and more resistant to antibodies. Mutants with large negative predicted ddG score were taken into the lab for experimental testing using surface plasmon resonance (SPR). Researchers utilized a Reichert4SPR to determine the binding of these mutants. Specifically, equilibrium binding constants for the homo-trimeric spike glycoprotein (S protein), and various predicted mutants, binding to hACE2 (human angiotensin-converting enzyme 2) were determined and compared to the predicted values.1
The first step in the virus cell infection process is cell entry, so blocking the binding of S protein to hACE2 could be key to virus transmission prevention. One way to accomplish that is to use monoclonal antibodies from convalescent plasma, which provides competition to the S protein binding and thus prevents it from binding. Another way is to develop a recombinant vaccine. A third possibility is to use a protein inhibitor.1
Mutations on the S binding protein of the RBD can change affinity to the cell receptor thus affecting potency of potential therapeutics. Researchers in this study note that 196 mutations in the RBD domain have been found. Also noted was the fact that while some mutants are less infectious, others are more infectious and tend to be resistant to neutralizing antibodies. These potentially more infectious mutants with higher affinity were the focus of this research with the aim of looking to future treatments.1
Researchers initially predicted protein-protein interaction strength for each mutant using the Rosetta Flex method, then determined the actual affinity with SPR to see how accurate their predictions were. A ddG value was calculated which indicates what the difference in affinity is once a protein mutates compared to the wild type.1
Researchers were interested in comparing SPR results with those predicted using an in-silico affinity maturation method. Their ultimate aim was to use in-silico methods to predict which mutants should be used for vaccine and other treatment development.1
In total, the Rosetta Flex method was applied to 475 possible single point mutants. Of those, 114 had a negative ddG score which indicated they might have higher affinity than the wild type. Mutants with a large negative ddG value were looked at manually and ultimately, 9 mutants were selected for further study using SPR. Researchers ran their SPR experiments as a kinetic titration, so no regeneration conditions needed to be determined. Of the 9 chosen, 6 targets showed improved binding to hACE2 (were higher affinity) compared to the wild type. The remaining 3 mutants showed either similar affinity as the wild type or lower. The authors reviewed the results and decided that hydrogen binding, which was not fully factored into the model for the three lower affinity binders, was the cause of the discrepancy for them.1
As complement, XanTec has developed a cross-platform, ready-to-use COVID-19 SPR sensor chip to meet the increasing demand for COVID-19-related research tools. The sensor chip consists of the SARS-CoV-2 receptor-binding domain (RBD) protein (wild-type) homogenously preimmobilized on XanTec’s proprietary ultra-low-background polycarboxylate HC surface. This new surface is a versatile tool for various applications in clinical and pharmaceutical COVID-19 R&D and available for various SPR instruments.
The C19RBDHC30M sensor chip was primarily developed for fast and label-free detection of anti-SARS-CoV-2 antibodies from patient serum. As the sample matrix contains serum and is thus prone to nonspecific interactions, a highly bioinert chip coating, optimized reagents, and an adapted protocol are essential for highly specific and sensitive detection of anti-SARS-CoV-2 antibodies2.