The identification of the optimal regeneration buffer is an integral part of a biomolecular interaction assay development. Regeneration conditions vary from ligand to ligand and require optimization in order to quantitatively remove the analyte without affecting the ligand's activity. This is the prerequisite to re-use the functionalized sensor surface.
All buffers are sterile filtered.
| Product description | Product code | Regeneration scouting kit 1 Scouting of optimal regeneration conditions, contains: - 0.01 M Glycine * HCl pH 1.5, 10 mL - 0.01 M Glycine * HCl pH 2.0, 10 mL - 0.01 M Glycine * HCl pH 2.5, 10 mL - 0.01 M Glycine * HCl pH 3.0, 10 mL - 0.05 M Sodium hydroxide, 10 mL - 4 M Magnesium chloride, 10 mL - 5 M Sodium chloride, 10 mL - 0.5% (w/v) SDS, 10 mL - 10% (v/v) Tween 20, 10 mL | K RK1-50I | Regeneration scouting kit 2 Scouting of optimal regeneration conditions, contains: - Regeneration cocktail - Acidic, 10 mL - Regeneration cocktail - Alkaline, 10 mL - Regeneration cocktail - Chelating, 10 mL - Regeneration cocktail - Detergent, 10 mL - Regeneration cocktail - Chaotropic, 10 mL - Regeneration cocktail - Non polar, 10 mL | K RK2-50I | Regeneration kit intensive Reagents for intensive regeneration of NiHC and NiD sensor chips - 5 M Guanidine thiocyanate, 5 mM TCEP, 50 mL - 1 mg/mL Pepsin in 1 M glycine * HCl pH 2.5, 50 mL - Conditioning buffer 1, 0.05 M NaOH, 1 M NaCl, 50 mL | K RIN-50I | Glycine buffer pH 1.5 Acidic regeneration buffer 0.01 M Glycine * HCl pH 1.5, 50 mL | B G15-50ML | Glycine buffer pH 2.0 Acidic regeneration buffer 0.01 M Glycine * HCl pH 2.0, 50 mL | B G20-50ML | Glycine buffer pH 2.5 Acidic regeneration buffer 0.01 M Glycine * HCl pH 2.5, 50 mL | B G25-50ML | Glycine buffer pH 3.0 Acidic regeneration buffer 0.01 M Glycine * HCl pH 3.0, 50 mL | B G30-50ML | 0.05 M Sodium hydroxide Alkaline regeneration solution 0.05 M NaOH, 50 mL | B NO-50ML | 5 M Sodium chloride High ionic strength regeneration solution 5 M NaCl, 50 mL | B SCl-50ML | 4 M Magnesium chloride Chaotropic regeneration solution 4 M magnesium chloride, 50 mL | B MC-50ML | 3 M Guanidine Chaotropic regeneration solution to regenerate Strep-Tactin XT/TST-binding complex 3 M Guanidine * HCl, 50 mL | B GU-50ML | 10% Tween 20 Detergent regeneration solution 10 % (v/v) Surfactant Tween 20, 50 mL | B T20-50ML | 0.5% SDS Detergent regeneration solution 0.5% (w/v) Sodium dodecyl sulphate (SDS), 50 mL | B SD-50ML | Ethylene glycol Regeneration solution Ethylene glycol p. a., 50 mL | B EG-50ML | Regeneration cocktail - Acidic Acidic cocktail for ligand regeneration 0.15 M Oxalic acid, 0.15 M H3PO4, 0.15 M formic acid, 0.15 M malonic acid pH 5.0, 50 mL | B RCA-50ML | Regeneration cocktail - Alkaline Alkaline cocktail for ligand regeneration 0.2 M Ethanolamine, 0.2 M Na3PO4, 0.2 M Piperazine, 0.2 M Glycine pH 9.0, 50 mL | B RCB-50ML | Regeneration cocktail - Chelating Chelating cocktail for ligand regeneration 0.02 M disodium EDTA, 50 mL | B RCC-50ML | Regeneration cocktail - Detergent Detergent cocktail for ligand regeneration 0.3% (w/v) CHAPS, 0.3% (w/v) Zwittergent 3-12, 0.3% (v/v) Tween 80, 0.3% (v/v) Tween 20, 0.3% (v/v) Triton X-100, 50 mL | B RCD-50ML | Regeneration cocktail - Chaotropic Chaotropic cocktail for ligand regeneration 0.46 M KSCN, 1.83 M MgCl2, 0.92 M Urea, 1.83 M Guanidine * HCl, 50 mL | B RCI-50ML | Regeneration cocktail - Non polar Non polar cocktail for ligand regeneration 20 % (v/v) DMSO, 20% (v/v) formamide, 20% (v/v) ethanol, 20% (v/v) acetonitrile, 20% (v/v) 1-butanol, 50 mL | B RCS-50ML |
|---|
*Andersson, Karl, Markku Hämäläinen, and Magnus Malmqvist. "Identification and optimization of regeneration conditions for affinity-based biosensor assays. A multivariate cocktail approach." Analytical chemistry 71.13 (1999): 2475-2481.