Products | Immobilization Kits, Buffers and Reagents | Regeneration buffers and kits

Regeneration buffers and kits

The identification of the optimal regeneration buffer is an integral part of a biomolecular interaction assay development. Regeneration conditions vary from ligand to ligand and require optimization in order to quantitatively remove the analyte without affecting the ligand's activity. This is the prerequisite to re-use the functionalized sensor surface.

Regeneration buffers and kits

All buffers are sterile filtered.

Product descriptionProduct code
Regeneration cocktail - Acidic, pH 5.0, 50 ml*.
Contains:
- 0.15 M Oxalic acid
- 0.15 M Phosphoric acid
- 0.15 M Formic Acid
- 0.15 M Malonic acid
B RCA-50
Regeneration cocktail - Alkaline, pH 9.0, 50 ml*.
Contains:
- 0.2 M Ethanolamine
- 0.2 M Sodium phosphate
- 0.2 M Piperazine
- 0.2 M Glycine
B RCB-50
Regeneration cocktail - Chelating, 50 ml*.
Contains:
- 20 mM EDTA
B RCC-50
Regeneration cocktail - Detergent, 50 ml*.
Contains:
- 0.3% (w/w) CHAPS
- 0.3% (w/w) Zwittergent 3-12
- 0.3% (w/w) Tween 80
- 0.3% (w/w) Tween 20
- 0.3% (w/w) Triton X-100
B RCD-50
Regeneration cocktail - Chaotropic, 50 ml*.
Contains:
- 0.46 M KSCN
- 1.83 M MgCl2
- 0.92 M Urea
- 1.83 M Guanidine-HCL
B RCI-50
Regeneration cocktail - Non-polar, 50 m*l.
Contains:
- 10 ml DMSO
- 10 ml Formamide
- 10 ml Ethanol
- 10 ml Acetonitrile
- 10 ml 1-Butanol
B RCS-50
Regeneration scouting kit 1*.
Contains:
- Glycine-HCl, 10 mM, pH 1.5, 10 ml
- Glycine-HCl, 10 mM, pH 2.0, 10 ml
- Glycine-HCl, 10 mM, pH 2.5, 10 ml
- Glycine-HCl, 10 mM, pH 3.0, 10 ml
- NaOH, 50 mM, 10 ml
- Magnesium chloride, 4.0 M, 10 ml
- 0.5% Sodium dodecyl sulphate (SDS), 10 ml
- 10% Surfactant P20 in H2O, 10 ml
- Ethylene glycol (p.a.), 10 ml
K RK1-50
Regeneration scouting kit 2*.
Contains:
- Regeneration cocktail - Acid, 10 ml
- Regeneration cocktail - Base, 10 ml
- Regeneration cocktail - Chaotropic, 10 ml
- Regeneration cocktail - Detergent, 10 ml
- Regeneration cocktail - Non-polar, 10 ml
- Regeneration cocktail - Chelating agent, 10 ml
K RK2-50
0,5% sodium dodecyl sulphate (SDS), 50 ml.B SD-50
Surfactant, T20, 10% Polyoxyethylenesorbitan in water, 50 ml.B T20-50
4.0 M magnesium chloride, 50 ml.B MC-50
NaOH, 50 mM, 50 ml.B NO-50
Regeneration buffer. Glycine * HCl, 10 mM, pH 1.5, 50 ml.B G15-50
Regeneration buffer. Glycine * HCl, 10 mM, pH 2.0, 50 ml.B G20-50
Regeneration buffer. Glycine * HCl, 10 mM, pH 2.5, 50 ml.B G25-50
Regeneration buffer. Glycine * HCl, 10 mM, pH 3.0, 50 ml.B G30-50

*Andersson, Karl, Markku Hämäläinen, and Magnus Malmqvist. "Identification and optimization of regeneration conditions for affinity-based biosensor assays. A multivariate cocktail approach." Analytical chemistry 71.13 (1999): 2475-2481.