All buffers are sterile filtered.
| Product description | Product code | Acetate buffer pH 4.0 Electrostatic preconcentration of protein ligands 5 mM sodium acetate pH 4.0, 50 mL | B A40-50ML | Acetate buffer pH 4.5 Electrostatic preconcentration of protein ligands 5 mM sodium acetate pH 4.5, 50 mL | B A45-50ML | Acetate buffer pH 5.0 Electrostatic preconcentration of protein ligands 5 mM sodium acetate pH 5.0, 50 mL | B A50-50ML | Acetate buffer pH 5.5 Electrostatic preconcentration of protein ligands 5 mM sodium acetate pH 5.5, 50 mL | B A55-50ML | Maleate buffer pH 6.0 Electrostatic preconcentration of protein ligands 2.5 mM sodium maleate pH 6.0, 50 mL | B M60-50ML | Amine coupling kit 1, standard Contains buffers and chemicals for 40 – 60 activations - solid EDC * HCl purity > 99 % SPR-grade, 1 g - Activation buffer, 0.05 M MES, 0.1 M NHS pH 5.0, 50 mL - Borate elution buffer, 0.1 M sodium borate, 1 M NaCl pH 9.0, 50 mL - Ethanolamine quenching buffer, 1 M Ethanolamine * HCl pH 8.5, 50 mL | K AN-50I | Amine coupling kit 2, sulfo-NHS Contains buffers and chemicals for 40 - 60 activations - solid EDC * HCl purity > 99 % SPR-grade, 1 g - Activation buffer S, 0.05 M MES, 0.1 M sulfo-NHS pH 5.0, 50 mL - Borate elution buffer, 0.1 M sodium borate, 1 M NaCl pH 9.0, 50 mL - Ethanolamine quenching buffer, 1 M Ethanolamine * HCl pH 8.5, 50 mL | K AS-50I | Amine coupling kit 3, zwitterionic surfaces Contains buffers and chemicals for approx. 30 activations of ZC surfaces: - solid EDC * HCl purity > 99 % SPR-grade, 1.5 g - Activation buffer, 0.05 M MES, 0.1 M NHS pH 5.0, 50 mL - 10×0.01 M MES immobilisation buffer pH 6.5, 50 mL - Borate elution buffer, 0.1 M sodium borate, 1 M NaCl pH 9.0, 50 mL - Quenching buffer A, 1 M glycine * HCl, 0.01 M MES, 0.01 % Thiomersal pH 6.5, 50 mL - Quenching buffer C, 1 M DMEN * HCl pH 6.5, 50 mL | K-AZ-30I | Thiol coupling kit Contains buffers and chemicals for 50 immobilizations of thiol-containing ligands via reactive surface disulfid groups (Amine coupling kit 1 required) - Coupling reagent, PDEA, 150 mg - Coupling buffer, 0.1 M sodium borate pH 8.5, 50 mL - Capping buffer, 0.05 M cysteine, 0.1 M sodium acetate, 1 M NaCl pH 4.0 | K TK-50I | DBCO labelling kit Contains buffers and chemicals for 3×DBCO conjugations, use with azide-modified sensor chips (AZD, AZHC, AZP) '- 3×DBCO-PEG4-NHS ester 0.13 mg - 3×ZEBA™Spin desalting columns 7 K MWCO, 0.5 mL - DMSO dry, 1 mL - 10×PBS buffer, 10×dilution yields: 0.01 M NaxHyPO4, 0.15 M NaCl pH 7.4, 50 mL - Ethanolamine quenching buffer, 1 M Ethanolamine * HCl pH 8.5, 5 mL | K DCL-3C | Borate elution buffer Preconditioning of Hydrogel coatings 0.1 M sodium borate, 1 M NaCl pH 9.0, 50 mL | B BELU-50ML | Borate elution buffer Preconditioning of hydrogel coatings 0.1 M sodium borate, 1 M NaCl pH 9.0, 500 mL | B BELU-500ML | Ethanolamine quenching buffer Quenching of active esters 1 M Ethanolamine * HCl pH 8.5, 50 mL | B EA85-50ML | Quenching buffer A Quenching of active esters on ZC sensor chips 1 M glycine * HCl, 0.01 M MES, 0.01 % Thiomersal pH 6.5, 50 mL | B QA65-50ML | Quenching buffer C Quenching of active esters on ZC sensor chips 1 M DMEN * HCl pH 6.5, 50 mL | B QC65-50ML | Activation buffer Activation of carboxylic acids 0.05 M MES, 0.1 M NHS pH 5.0, 50 mL | B MN-50ML | Activation buffer S Activation of carboxylic acids 0.05 M MES, 0.1 M sulfo-NHS pH 5.0, 50 mL | B MSN-50ML | EDC * HCl Activation of carboxylic acids solid EDC * HCl purity > 99 % SPR-grade, 1 g | C EDCHCL-1G | EDC * HCl Activation of carboxylic acids Solid EDC * HCl purity > 99 % SPR-grade, 5×1 g | C EDCHCL-5X1G | Buffer kit for NiD and NiHC sensor chips Contains buffers and reagents for approx. 50 immobilisations: - 10×HBSTE running buffer, 10×dilution yields: 0.01 M HEPES, 0.15 M NaCl, 0.005 % Tween 20, 50 µM EDTA pH 7.4, 50 mL - NiCl2 loading buffer, 5 mM NiCl2 in HBSTE running buffer, 50 mL - EDTA regeneration buffer, 0.35 M EDTA trisodium salt pH 8.5, 50 mL - Elution buffer, 0.01 M NaOH, 1 M NaCl pH 12, 50 mL | K NI-50I | NiCl2 loading buffer Loading of NiHC, NiD and NiP sensor chips with Ni2+-ions, part of K NI-50I 5 mM NiCl2 in HBSTE running buffer, 50 mL | B NILB-50ML | EDTA regeneration buffer Regeneration of NiHC, NiD and NiP sensor chips loaded with Ni2+-ions, part of K NI-50I 0.35 M EDTA trisodium salt pH 8.5, 50 mL | B EDTA035-50ML | Elution buffer Elution of NiHC, NiD and NiP sensor chip bound proteins, part of K NI-50I 0.01 M NaOH, 1 M NaCl pH 12, 50 mL | B ELU12-50ML | RG-modifier Azide terminated oligonucleotide (RG-MOD) for direct modification of DBCO-labelled ligands, use with RGD sensor chips, sufficient for approx. 50 injections 100 µM (3 nmol) RG-MOD in 0.01 M HEPES, 0.1 M EDTA pH 7.0, 30 µL stock | C RG-MOD-3NM | RG biotin capture reagent Oligonucleotide-modified streptavidin conjugate (RG-SA) for reversible immobilisation of biotinylated ligands on RGD sensor chips, sufficient for approx. 50 injections 10 µM RG-SA (1.5 nmol), 100×dilution yields 15 mL of 100 nM RG-SA working solution, 150 µL stock | C RG-SA-50I | Conditioning buffer 1 Preconditioning of Streptavidin, Neutravidin, Streptactin XT and RG sensor chips 0.05 M NaOH, 1 M NaCl, 50 mL | B C1-50ML |
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