Heparin–modified sensor chips
XanTec’s heparin (HEP) sensor chips are based on a 50 nm heparin hydrogel matrix grafted onto a hydrophilic adhesion promoter on a gold support. Ligands can be covalently attached via their amine, thiol, or aldehyde groups using standard coupling chemistries such as EDC/NHS activation, thiol–maleimide conjugation, or reductive amination. Efficient protein immobilization requires electrostatic preconcentration prior to covalent coupling.
Although heparin surfaces are not typically the first choice for routine kinetic or affinity analyses, they provide a valuable alternative when CMD- or HC-based sensor chips are unsuitable. In addition, HEP sensor chips serve as benchmark coatings for assessing nonspecific binding during the development and evaluation of biomedical or antifouling surface chemistries, particularly in applications focused on hemocompatibility.
Key features:
- Alternative to CMD or HC surfaces: Provides a 3D hydrogel matrix for covalent ligand immobilization when CMD or HC coatings are unsuitable.
- Versatile ligand coupling: Supports covalent attachment through amine, thiol, or aldehyde groups using established chemistries (EDC/NHS, maleimide, reductive amination).
- Benchmark surface: Useful as a reference coating for evaluating nonspecific binding and assessing new biomedical coating materials.
| Product code | HEP |
|---|---|
| Base coating | 3D, 50 nm heparin (medium density) |
| Electrostatic preconcentration capacity [µRIU]2 | ≈ 11,000 |
| Recommended ligands |
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| Recommended analytes |
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| Intended purpose |
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1 All illustrations are schematic representations and are not drawn to scale; dimensions, densities, and spatial relationships do not reflect actual physical or chemical proportions.
2 Preconcentration capacity determined by injecting 100 µg/mL BSA in 5 mM sodium acetate pH 5.0, with 1 µRIU corresponding approximately to 1 RU. Maximum covalent coupling yields depend strongly on ligand properties and typically range from approximately 20–45% of the respective electrostatic preconcentration capacity.