C-type sensor chips
XanTec’s C-type sensor chips are based on a synthetic 3D hydrogel matrix composed of linear aliphatic polycarboxylate chains with very high charge density, grafted onto a hydrophilic adhesion promoter on a gold support. Ligands can be covalently attached via their amine, thiol, or aldehyde groups using established coupling chemistries such as EDC/NHS activation, thiol–maleimide conjugation, or reductive amination. This enables immobilization of proteins, antibodies, peptides, nucleic acids, and small molecules.
The exceptionally high charge density renders the surface susceptible to nonspecific binding of cationic biomolecules; however, the aliphatic polymer backbone provides outstanding chemical robustness. This makes C-type coatings particularly well suited for demanding chemical environments and for non-biological applications that require a dense and chemically well-defined carboxylate (COOH) surface, such as solid-phase syntheses or the preparation of metal–organic frameworks (MOFs).
Key features:
- Exceptional chemical stability: The aliphatic polymer backbone provides the highest chemical robustness within XanTec’s portfolio, enabling use under harsh chemical conditions and supporting non-biological applications requiring a dense COOH surface.
- Very high carboxyl group density: Enables highly efficient ligand immobilization under electrostatic preconcentration conditions.
- Versatile coating thickness: Available in thicknesses from 30 to 150 nm, allowing flexible adaptation to specific experimental requirements.
| Product code | C30M | C80M | C150D |
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| Base coating | 3D, 30 nm aliphatic polycarboxylate (medium density) |
3D, 80 nm aliphatic polycarboxylate (medium density) |
3D, 150 nm aliphatic polycarboxylate (high density) |
| Electrostatic preconcentration capacity [µRIU]2 | ≈ 9,000 | ≈ 23,000 | ≈ 50,000 |
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1 All illustrations are schematic representations and are not drawn to scale; dimensions, densities, and spatial relationships do not reflect actual physical or chemical proportions.
2 Preconcentration capacity determined by injecting 100 µg/mL bovine serum albumin (BSA) in 5 mM sodium acetate pH 5.0, with 1 µRIU corresponding approximately to 1 RU. Maximum covalent coupling yields depend on ligand properties and typically range from approximately 25–45% of the respective electrostatic preconcentration capacity.